Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella Citation
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چکیده
Citation Cao, Bo, Qiuxiang Cheng, Chen Gu, Fen Yao, Michael S. DeMott, Xiaoqing Zheng, Zixin Deng, Peter C. Dedon, and Delin You. “ Pathological Phenotypes and in Vivo DNA Cleavage by Unrestrained Activity of a Phosphorothioate-Based Restriction System in S Almonella .” Molecular Microbiology 93, no. 4 (July 23, 2014): 776–785. As Published http://dx.doi.org/10.1111/mmi.12692 Publisher Wiley Blackwell
منابع مشابه
Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella.
Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction-modification (R-M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R-M systems, highly partial PT modification of consensus motifs in bacterial genomes suggests an unusual mechanism of PT-dependent restriction. In Salmonella ente...
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A method for achieving strand specific nicking of DNA has been developed. Phosphorothioate groups were incorporated enzymatically into the (-)strand of M13 RF IV DNA. When such DNA is reacted with restriction endonucleases in the presence of ethidium bromide nicked DNA (RF II) is produced. All of the restriction enzymes tested linearised phosphorothioate-containing DNA in the absence of this dy...
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The effects of nucleotide analogue substitution on the cleavage efficiencies of type II restriction endonucleases have been investigated. Six restriction endonucleases (EcoRV, SpeI, XbaI, XhoI, PstI and SphI) were investigated respectively regarding their cleavage when substrates were substituted by 2'-O-methyl nucleotide (2'-OMeN) and phosphorothioate (PS). Substitutions were made in the recog...
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